Search results for " UHPLC"

showing 10 items of 10 documents

Universal method to determine acidic licit and illicit drugs and personal care products in water by liquid chromatography quadrupole time-of-flight

2016

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emerging contaminantsPersonal careChromatographyChemistrywater010401 analytical chemistryClinical Biochemistry010501 environmental sciencesMass spectrometryHigh resolution mass spectrometry01 natural sciencesquantificationNon-target screening by SPE and UHPLC quadrupole time-of-flight0104 chemical sciencesMedical Laboratory TechnologyLiquid chromatography–mass spectrometryEnvironmental ScienceUHPLCidentificationQuadrupole time of flightComputingMethodologies_COMPUTERGRAPHICS0105 earth and related environmental sciencesMethodsX
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Exploring the apparent properties and Q-markers for quality control of Cornus officinalis

2023

Cornus officinalis Siebold & Zucc. has a high nutritious value and is widely used in food fields. To distinguish the quality of cornus in different areas, this research provided a new strategy for comprehensively evaluating cornus. As a result, 12 iridoid glycosides and 7 polyphenols were identified and quantified by UHPLC-Q-TOF/MS in 13 batches of samples from five different origins. Chromaticity analysis pointed out that pelargonidin-3-O-galactoside, total iridoids, and total polyphenols performed high relation with 1365*. Antioxidant activity and inhibition of α-glucosidase effect correlated with the chemical analysis suggested that iridoid glycosides and polyphenols should represent…

Cornus officinalis Siebold &ampZucc. (Cornaceae) UHPLC-Q-TOF/MS Q-Marker Purification ChromaticitySettore BIO/03 - Botanica Ambientale E ApplicataGeneral MedicineFood ScienceAnalytical Chemistry
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Deficit irrigation and maturation stage influence quality and flavonoid composition of ‘Valencia’ orange fruit

2016

BACKGROUND:Effects of continuous deficit irrigation (DI) and partial rootzone drying (PRD) treatments (50%ETc) in comparison with full irrigation (CI, 100% ETc) were investigated during ‘Valencia’ orange fruit maturation. Ultra-high-performance liquid chromatography/high-resolutionmass spectrometry was used to quantify hesperidin, narirutin, tangeritin, nobiletin, didymin and neoeriocitrin in the fruit juice and peel. RESULTS: No significant effect of irrigation was found on yield, juice soluble solids or acidity. Juice color was not influenced by irrigation or harvest date, whereas peel color increased during maturation and was more pronounced in CI and PRD fruits. Juice acidity reached a …

partial rootzone dryingSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeUHPLC/HESI-MShesperidinSettore CHIM/10 - Chimica Degli AlimentiCitrus sinensis; hesperidin; partial rootzone drying; peel color; soluble solids; UHPLC/HESI-MSpeel colorCitrus sinensisoluble solid
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A new multi analytical approach for the identification of synthetic and natural dyes mixtures. The case of orcein-mauveine mixture in a historical dr…

2017

In this paper, the application of a multi-analytical approach for the characterisation of synthetic and natural dyes in a historical textile is presented. The work is focused on a historical dress of a Sicilian noblewoman, dating from about 1865–1870. Firstly, SERS on fibre was performed, in order to individuate the classes of dyes employed. The SERS spectra suggested the presence of two main dyes: mauveine and orcein. In order to confirm these preliminary results, two different extraction protocols were applied. The extracts obtained were analysed by ESI-MS, MALDI-ToF and UHPCL-MS analyses, confirming the SERS results. In particular, the application of the ammonia mild extraction technique…

Historynew ammonia extraction methodComplex Mixtureorcein dyeMALDI-ToF; Mauveine; new ammonia extraction method; orcein dyes; SERS on fibre; Sicilian dress; UHPLC-MS; Clothing; Coloring Agents; Complex Mixtures; Female; History 19th Century; Humans; Oxazines; Sicily; Spectrum Analysis; TextilesMALDI-ToF; Mauveine; new ammonia extraction method; orcein dyes; SERS on fibre; Sicilian dress; UHPLC-MS; Analytical Chemistry; Biochemistry; Plant Science; Organic ChemistryMauveineSicilian dreSERS on fibreOxazineColoring agentsPlant ScienceComplex Mixtures01 natural sciencesBiochemistrylaw.inventionAnalytical ChemistryUHPLC-MSClothingUhplc mschemistry.chemical_compoundlawMauveineOxazinesHumansColoring AgentsColoring AgentOrceinSicilySpectrum AnalysiMALDI-ToF19th CenturyChromatographyPolymer science010405 organic chemistryChemistrySpectrum AnalysisTextilesOrganic Chemistryorcein dyesHistory 19th CenturySettore CHIM/06 - Chimica Organica0104 chemical sciencesSicilian dress010404 medicinal & biomolecular chemistryFemaleSpectrum analysismauveine; orcein dyes; Sicilian dress; new ammonia extraction method; SERS on fibre; MALDI-ToF; UHPLC-MSHuman
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Phenolic Compounds Characterization and Antioxidant Properties of Monocultivar Olive Oils from Northeast Algeria

2020

In Algeria, the olive tree is one of the main fruit species and plays a very important socioeconomic role. The objective of this study was firstly, to identify and quantify the phenolics of some Algerian olive oils, and secondly, to assess the antioxidant activity of the samples. The olive oils used in this study were derived from Algerian cultivars, including Tefahi, Gelb Elfarroudj, Chemlal, and imported cultivar Manzanilla and Zebboudj. For this purpose, gas chromatography&mdash

Antioxidant030309 nutrition & dieteticssecoiridoidsphenolicsmedicine.medical_treatmentPlant ScienceGelb elfarroudj cultivar; Manzanilla; Phenolics; Radical scavenging; Secoiridoids; Tefahi; UHPLC-HESI-MS03 medical and health sciencesHuman healthUHPLC-HESI-MS0404 agricultural biotechnologymedicineFood scienceCultivarlcsh:Agriculture (General)Volume concentrationradical scavenging0303 health sciencesChemistryManzanilla; Tefahi; Gelb Elfarroudj cultivar; secoiridoids; radical scavenging; UHPLC-HESI-MS phenolicsfood and beverages04 agricultural and veterinary sciencessecoiridoidlcsh:S1-972040401 food scienceManzanillaGelb Elfarroudj cultivarAntioxidant capacityOil productionComposition (visual arts)TefahiAgronomy and Crop ScienceFood ScienceOlive oilAgriculture
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Metabolic signatures across the full spectrum of non-alcoholic fatty liver disease.

2022

Funder: European Commission

ALTtype 2 diabetes mellitusROC receiving operator characteristicaspartate aminotransferaseHSDLDL low-density lipoproteinUHPLC ultrahigh-performance liquid chromatographyROCHCCNon-alcoholic steatohepatitisGCPCANASHGastroenterology2-HB 2-hydroxybutanoic acid; 3-HB 3-hydroxybutanoic acid; ALT alanine aminotransferase; AST aspartate aminotransferase; CE cholesterol ester; Cer ceramide; FFA free fatty acid; FLIP Fatty Liver Inhibition of Progression; Fibrosis; GC gas chromatography; HCC hepatocellular carcinoma; HSD honest significant difference; LC lipid cluster; LDL low-density lipoprotein; LM lipid and metabolite; LMC lipid metabolite and clinical variable; LPC lysophosphatidylcholine; Lipidomics; Mass spectrometry; Metabolomics; NAFL non-alcoholic fatty liver; NAFLD non-alcoholic fatty liver disease; NAS NASH activity score; NASH non-alcoholic steatohepatitis; NIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research Network; NRR non-rejection rate; Non-alcoholic steatohepatitis; PC(O) ether PC; PC phosphatidylcholine; PCA principal component analysis; PE phosphatidylethanolamine; QTOFMS quadrupole-time-of-flight mass spectrometry; ROC receiving operator characteristic; SAF steatosis activity and fibrosis; SM sphingomyelin; T2DM type 2 diabetes mellitus; TG triacylglycerol; UHPLC ultrahigh-performance liquid chromatographySAFSAF steatosis activity and fibrosisLM lipid and metabolitehonest significant differenceHSD honest significant differenceTG triacylglycerolnon-rejection ratecholesterol esterPCPEGC gas chromatographyfree fatty acidFLIPNASH non-alcoholic steatohepatitisNIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research NetworkBIOMARKERST2DMPE phosphatidylethanolamineLDLlipidNAFLDFFA free fatty acid2-HBMetabolomicsNAFL non-alcoholic fatty liverLMCphosphatidylcholineScience & TechnologySM sphingomyelinGastroenterology & HepatologyMass spectrometryactivitynutritional and metabolic diseasesT2DM type 2 diabetes mellitusACIDSreceiving operator characteristicdigestive system diseasesquadrupole-time-of-flight mass spectrometryLC lipid clusterlow-density lipoproteinNAS2-HB 2-hydroxybutanoic acidNAS NASH activity scoreQTOFMSether PCNRRSCORING SYSTEMprincipal component analysisgas chromatographyLC2-hydroxybutanoic acidPROGRESSIONAST aspartate aminotransferaseLMPC phosphatidylcholinePC(O)MARKERSUHPLCsteatosisTOOLImmunology and AllergyINSULIN-RESISTANCECerSMFatty Liver Inhibition of Progressionhepatocellular carcinoma2-HB 2-hydroxybutanoic acid NIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research Network NRR non-rejection rate Non-alcoholic steatohepatitis PC(O) ether PC PC phosphatidylcholine PCA principal component analysis PE phosphatidylethanolamine QTOFMS quadrupole-time-of-flight mass spectrometry ROC receiving operator characteristic SAF steatosis activity and fibrosis SM T2DM type 2 diabetes mellitus TG triacylglycerol UHPLC ultrahigh-performance liquid chromatographyultrahigh-performance liquid chromatographyCELPC3-HBNAFLnon-alcoholic fatty liverTGtriacylglycerolNRR non-rejection rateLife Sciences & BiomedicineNAFLD non-alcoholic fatty liver diseaseFLIP Fatty Liver Inhibition of Progressionalanine aminotransferasemetaboliteCer ceramideCE cholesterol estersphingomyelinlysophosphatidylcholineand fibrosisALT alanine aminotransferaseInternal MedicineceramideNational Institute of Digestive Diseases and Kidney NASH Clinical Research NetworkAST3-HB 3-hydroxybutanoic acidQTOFMS quadrupole-time-of-flight mass spectrometryPCA principal component analysisLPC lysophosphatidylcholineHepatologynon-alcoholic fatty liver diseaseand clinical variablePC(O) ether PC3-hydroxybutanoic acidFibrosisNASH activity scoreNIDDK NASH-CRNlipid clusterlipid and metabolitephosphatidylethanolamineLipidomicsLMC lipid metabolite and clinical variableFFAHCC hepatocellular carcinomaJHEP reports : innovation in hepatology
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Analysis of β2-agonists in cattle hair samples using a rapid UHPLC–ESI–MS/MS method

2016

A simple and efficient method was developed for simultaneous analysis of five illegal residual β2-agonists in cattle hair. β2-Agonists were quantified by ultra high performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry operating in positive multiple-reaction monitoring mode. The method was validated as quantitative confirmatory method according to the EU Decision 2002/657/EC: instrumental linearity, specificity, precision, recovery, decision limit (CCα) and detection capability (CCβ) were evaluated. The recovery were greater than 90% and the method appeared suitable for the control of these β2-agonists in cattle hair samples with LOQ values between …

UHPLC –ESI–MS/MSβ2-agonist0301 basic medicineElectrospray030109 nutrition & dieteticsChromatographycattle hair; Commission Decision 2002/657/EC; UHPLC–ESI–MS/MS; β2-agonists; Plant Science; Biochemistry; Analytical Chemistry; Organic ChemistryChemistryβ2 agonistsElectrospray ionization010401 analytical chemistryOrganic ChemistryPlant ScienceContaminationTandem mass spectrometry01 natural sciencesBiochemistry0104 chemical sciencesAnalytical Chemistry03 medical and health sciencesCommission Decision 2002/657/ECcattle hairAdrenergic beta-AgonistsUltra high performanceDecision limitNatural Product Research
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Quality evaluation of extra-virgin olive oils from Sicilian genotypes grown in a high-density system

2019

Studying the sensory profile and chemical composition of monovarietal extra-virgin olive oils (EVOOs) is important to define and manage their quality and uniqueness. Chemical and sensory traits of olive oils from 14 minor Sicilian olive genotypes in comparison with oils from six major Sicilian and three international cultivars were analysed. Oils were extracted in 2015 from fruit of the 23 genotypes grown in an experimental orchard at a planting density of 1140 trees ha−1. Fatty acid composition, phenol composition, carotenoid content and antioxidant power were determined and analysed using univariate and multivariate procedures, in particular Nocellara Etnea along with carotenoid, phenol c…

Chlorophyll0301 basic medicineSettore CHIM/10 - Chimica Degli AlimentiGenotypeSettore AGR/13 - Chimica Agraria030209 endocrinology & metabolismSensory profileBiologyAntioxidants03 medical and health sciences0302 clinical medicinePhenolsOleaPlant OilsCultivarOlive OilCarotenoidChemical compositionCarotenoids chlorophyll fatty acids phenols sensory attributes UHPLC-HESI-MSchemistry.chemical_classification030109 nutrition & dieteticsFatty Acidsfood and beveragesSowingCarotenoidslanguage.human_languageSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeHorticulturechemistryFruitTastelanguageComposition (visual arts)OrchardSicilianFood ScienceInternational Journal of Food Sciences and Nutrition
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Ultra-high-pressure liquid chromatography tandem mass spectrometry method for the determination of 9 organophosphate flame retardants in water samples

2016

Few methods are available for comprehensive organophosphate flame retardants (PFRs) detection in water and wastewater. Gas chromatography has been employed previously, but this approach is less selective, not amenable for use with deuterated standards and can suffer unfavorable fragmentation. Ultra-high-pressure liquid chromatography tandem mass spectrometry (UHPLC-QqQ-MS/MS) has become the most promising platform, already applied successfully for analysis of selected PFRs in some environmental matrices like water and wastewater. However, the presence of some interferences from the dissolvent, the equipment and the used materials should be taken into account. The procedure involves: The fir…

ChromatographyChemistry010401 analytical chemistryClinical BiochemistryAnalytical chemistryAnalysis of organophosphate flame retardants in water by SPE and UHPLC–MS/MS using a trap column.solid phase extractionWater010501 environmental sciences01 natural sciencesTrap column0104 chemical sciencesOrganophosphate flame retardantsMedical Laboratory TechnologyWastewaterLC–MS/MSLiquid chromatography–mass spectrometryInterferencesLc ms msEnvironmental ScienceGas chromatographyUltra high pressureSolid phase extraction0105 earth and related environmental sciencesComputingMethodologies_COMPUTERGRAPHICSMethodsX
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Untargeted screening of the bound / free phenolic composition in tomato cultivars for industrial transformation

2019

BACKGROUND Tomato is one of the most important agricultural crops and it is characterized by a wide bioactive compound profile. However, little information is reported on its comprehensive polyphenol profile. In this work, 13 commercial tomato cultivars for industrial transformation were screened by ultra-high-pressure liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UHPLC-QTOF-MS) for both free and bound phenolic profiles. Thereafter, the in vitro antioxidant activity of each cultivar was assessed by ferric reducing antioxidant power (FRAP) and oxygen radical absorbance activity (ORAC) assays. Multivariate statistics, i.e. orthogonal projection to latent struct…

Hydroxybenzoic acidAntioxidantfood metabolomicsin vitro antioxidant activitymedicine.medical_treatmentbound phenolicsfood qualityFlavonesAntioxidantsMass SpectrometryAnthocyaninsAbsorbancechemistry.chemical_compoundSolanum lycopersicumPhenolsUHPLC-QTOF-MSSettore AGR/13 - CHIMICA AGRARIAmedicineCultivarFood scienceChromatography High Pressure LiquidpolyphenolsFlavonoidschemistry.chemical_classificationNutrition and DieteticsPlant ExtractsChemistryfungifood and beveragesBioactive compoundPolyphenolFruitComposition (visual arts)Agronomy and Crop Sciencebound phenolics ; polyphenols ; food metabolomics ; food quality ; in vitro antioxidant activity ; UHPLC-QTOF-MSFood ScienceBiotechnology
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